Fig. 7

The FBW7/GSK3β/IGF2BP2/SLC7A5 axis modulates radiosensitivity in lung cancer. A and B H1299, A549, and H1299-RR cells were infected with indicated lentivirus. After puromycin selection, cells were harvested for Western blotting analysis. C H1299, A549, and H1299-RR cells were infected with indicated lentivirus. After puromycin selection, cells were irradiated at various doses for clonogenic survival assay. n = 3, one-way ANOVA. Data are presented as Mean ± SD. D and E H1299, A549, and H1299-RR cells were infected with indicated lentivirus. After puromycin selection, cells were irradiated at 6 Gy. After 4 h, cells were harvested for neutral comet assay. n = 3, one-way ANOVA. Data are presented as Mean ± SD. Scale bar, 50 μm. F A549 cells were transfected with indicated constructs. After puromycin selection, cells were injected subcutaneously into nude mice. The mice were treated with or without IR (10 Gy). Tumor volumes were measured every 3 days. Tumors were harvested, photographed, and weighted at day 21. n = 5, one-way ANOVA. Data are presented as Mean ± SD. G The schematic diagram depicting that FBW7 together with GSK3β promote IGF2BP2 degradation, thus inhibit the IGF2BP2-SLC7A5 feedback loop and its effect on promoting lung cancer radioresistance. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Abbreviations: BP2, IGF2BP2; F7, FBW7