Fig. 1

PARP1 expression along the BE to EAC transition in patient samples and in the L2-IL1B mouse model. A Human endoscopic submucosal resection samples (n = 14) were annotated according to their histopathology stage on HE-stained sections and PARP1 expression was analyzed within each category. In the displayed example, normal squamous epithelium (green), BE without high-grade Intraepithelial Neoplasia/IEN (white), BE with IEN (blue) and EAC (yellow) were present. B Quantification of the % PARP1 positive tissue area (pta) of all recorded 20 × fields-of-view and the mean value per category. * p < 0.05, ** p < 0.01, **** p < 0.0001 (Kruskal–Wallis-Test with Dunn’s correction for multiple comparisons). C Representative IHC images from the squamocolumnar junction (SCJ) of WT and L2-IL1B mice. In L2-IL1B mice, epithelial dysplastic cells (blue arrows) and inflammatory lymphocytes (orange arrows) were positive for PARP1 compared with WT mice, which did not present dysplastic epithelium and/or inflammation. D Quantification of PARP1 expression in murine SCJ tissues as % pta. PARP1 was significantly more expressed in dysplasia lesions graded 2 (p < 0.0001) and 3 (p = 0.0006) than normal areas without dysplasia (grade 0). Data are represented as single individually plotted values per field (3 WT mice and 2 to 4 L2-IL1B mice per group) and mean ± SEM. *** p < 0.001 and **** p < 0.0001 by one-way ANOVA followed by posthoc Tukey’s test