Fig. 2

PARP1 expression and PARPi-FL binding in human and murine organoids. A Schematic of procedure of the isolation of organoids from patient biopsies and staining with PARPi-FL for confocal microscopy. B Representative PARP1 IHC of human BE organoids. Consecutive z-stack images (step size 0.6 µm) of a representative human (C) or murine (D) organoid incubated with 0.5 µM PARPi-FL for 10 min. Green fluorescence = PARPi-FL