Fig. 3

Ex vivo wide-field imaging showed PARPi-FL accumulation in dysplastic lesions at the squamocolumnar junction (SCJ) of L2-IL1B mice. A Representative white light and fluorescence ex vivo images of the whole excised stomach of a PBS-injected control (WT mouse) and two PARPi-FL injected L2-IL1B mice and confocal microscopy of dysplastic lesions at the SCJ. Black arrows point to macroscopically visible lesions in the L2-IL1B mice. White dotted lines outline the SCJ and white squares are enlarged in the close-up image. B Quantification of PARPi-FL-related fluorescence of all lesions at the SCJ and background fluorescence intensity of each PARPi-FL-injected L2-IL1B mouse (single lesions and mean plotted). C Tumor-to-background ratio (TBR) of individual lesions in PARPi-FL-injected animals. Data are represented as single plotted values of each lesion ROI and mean ± SEM per mouse. D An incidental lesion at the esophagus showed similar PARPi-FL uptake (MFI ~ 15,810) to the SCJ