Fig. 4
PARPi-FL enabled lesion detection in L2-IL1B/IL8Tg mice with dysplastic lesions by fluorescence molecular endoscopy (FME). A Representative endoscopy frames of PARPi-FL injected and non-injected mice of different scorescomb. Gray-scale and intensity-coded false color images of selected frames are displayed. Displayed gray-scale images were normalized to the background (BG) by subtracting the average BG value from each whole image. Individual lesions are highlighted (orange dotted line). B Quantification of TBRs of all individually located lesions in PARPi-FL-injected mice by FME with mean ± SEM. C Quantification of the mean TBR of all lesions per mouse in PARPi-FL-injected mice with mean ± SEM. D Comparison of mean fluorescence intensity (MFI) measured in non-injected mice (n = 2) and the detected lesions in PARPi-FL-injected mice (n = 12). One non-injected mouse was excluded from the endoscopy quantification due to errors in setting acquisition parameters. In non-injected mice, the regions with the highest fluorescence detected by the endoscopy system were considered as a comparison. The quantification of the fluorescence was performed blindly. Significance levels were determined by one-way ANOVA followed by Dunnett’s multiple comparison test. * p < 0.05, ** p < 0.01 and **** p < 0.0001