Fig. 2

TRIM25 knockdown inhibits GBM proliferation, migration, and invasion in vitro. A Western blotting analysis was used to assess TRIM25 knockdown efficiency in LN229 and U251 cells transfected with TRIM25 siRNAs. B Western blotting analysis was used to assess TRIM25 knockdown efficiency in LN229, U251 and patient-derived GBM#021 cells transfected with TRIM25 shRNAs. C EdU assays of transfected LN229 and U251 cells. Graphical representation of EdU-positive cells in cultures of LN229- and U251-si-NC, si-TRIM25-1, and si-TRIM25-2 cells. Scale bars = 100 μm. D Growth curves for TRIM25-knockdown LN229 and U251 cells generated by cell counting over 72 h. E Representative images of fixed and stained Transwell migration and invasion assays involving transfected LN229 and U251 cells. Scale bars = 100 μm. F Representative images of 3D tumor spheroid invasion assays and quantification of invasion area. Scale bars = 500 μm. ns: not significant, ∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗: p < 0.001, and ∗∗∗∗: p < 0.0001