Fig. 1
An association between m6A and digestive tract tumors was revealed. (A-F) from M6A mRNA levels in 5 pairs of clinical tumor tissues (colorectal, esophageal, gastric, pancreatic, hepatocellular, and gallbladder cancers) with adjacent normal samples. (G) mRNA levels of m6A components were detected in 5 pairs of CRC tissues and adjacent tissues. (H) After ZCCHC4 was knocked down, the m6A content in each CRC cell line was detected to decrease accordingly. (I) The expression of ZCCHC4 was verified by immunohistochemical staining in CRC and adjacent tissues. (J) ZCCHC4 mRNA level were detected by qRT-PCR in 50 consecutive CRC cohorts. (K) The protein expression of ZCCHC4 in 3 pairs of CRC and adjacent tissues was verified by western blot