Fig. 2

ZCCHC4 is associated with CRC prognosis and the biological behavior of CRC cells in vitro. (A) The relationship between overall survival and ZCCHC4 expression was analyzed in 238 consecutive cases of CRC. (B) The bar graph describes the ZCCHC4 mRNA levels of different CRC cell lines compared with normal intestinal mucosal epithelial cell lines (NCM-460). (C) Expression levels of ZCCHC4 protein in different CRC cell lines Transwell tests (D), CCK-8 (E, F) and wound healing tests (G) demonstrated that down-regulation of ZCCHC4 inhibited the proliferation, migration and invasion of CRC cells, while up-regulation of ZCCHC4 induced the opposite effect. Flow cytometry down-regulated ZCCHC4 in HCT-116 (H) and HCT-15 (I) cells to block the cell cycle in G1 phase