Fig. 6

PTEN protein expression exhibits a positive correlation with USP8 expression levels both in vivo and at the single-cell dimension. A PTEN and USP8 expression in BBN-treated mouse urothelium were assessed via immunohistochemistry (IHC). B-C Time-dependent analysis of PTEN and USP8 expression levels in BBN-treated mouse bladder urothelium, denoted by integrated optical density (IOD); * p < 0.05, **p < 0.01, ****p < 0.0001. D Correlation between PTEN and USP8 expression levels in BBN-treated mouse urothelium over a period ranging from 4 to 18 weeks, measured by the Pearson coefficient (r). E–H PTEN and USP8 expressions were evaluated in tumor tissues from athymic nude mice injected with SNHG1-overexpressed cells and corresponding vector scramble transfectants (E). Their expression levels were quantified and depicted by IOD/area (F-G), and the correlation between PTEN and USP8 was calculated using the Pearson coefficient (H). Bars represent the mean ± SD for each group. Student’s t-test was employed to determine the p-value, with an asterisk (*) indicating a significant increase compared to Vector transfectants (p < 0.05). I The correlation between PTEN and USP8 in 393 cells from BC tissues was analyzed (GSE135337, BC single-cell RNA-seq)