Fig. 2

PARD3 overexpression accelerated liver cancer progression. PARD3 overexpression resulted in significantly higher hepatic (A) tumour burden, (B) tumour incidence, (C) tumour multiplicity and (D) tumour volume in CDAA diet- and MCD diet-fed mice. (E) Tumours derived from PARD3-overexpressing Hepa1-6 cells exhibited accelerated progression in the orthotopic liver cancer model. (F) PARD3 overexpression resulted in increased lung metastasis. (G) H&E staining on the liver tumour sections revealed a more pronounced invasiveness at the tumour periphery in PARD3 overexpression orthotopic HCC. (H) Higher CD31 and Ki67 expression was observed in PARD3-overexpressing Hepa1-6 orthotopic tumour tissue sections. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., not statistically significant