Fig. 6

Berberine suppressed HCC tumorigenesis by inhibiting PARD3 expression. (A) Berberine suppressed PARD3 expression in the liver. Restoration of PARD3 expression abolished the effects of berberine and increased the (B) tumour incidence, (C) tumour multiplicity, (D) tumour volume and (E) serum AFP level. F&G. Recovery of PARD3 effectively abolished the inhibitory effects of berberine on Ki67 and CD133, as measured by immunohistochemical analysis. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., not statistically significant