Fig. 2

IGF-1R promotes bone metastasis of NPC. a IGF-1R overexpression in 5-8F and knockdown in BM3. b CCK8 assay of cell lines to evaluate cell viability. c BLI quantitation of tumor burden of the mice. d Representative images of BLI of whole bodies. e BLI analysis of femurs. f Representative micro-CT images of femurs. g Micro-CT analyses for bone destruction of femurs. h H&E representative images and quantitative analysis of bone metastasis. (Tumor tissue is noted in the red line, “ns” indicates P > 0.05, “*” represents P < 0.05, “**” represents P < 0.01,“***” represents P < 0.001 and “****” represents P < 0.0001, Scar bar = 100 μm.)