Fig. 5
The mTORC1 inhibition suppresses IGF-1R–induced tumor proliferation and bone metastasis. a The protein levels of S6, pS6, IGF-1R and pIGF-1R in 5-8F, 5-8F OE and BM3 cells cultured in osteoclastic CM or routine medium with recombinant IGF-1 after rapamycin treatment. b CCK8 assay of cell lines cultured in osteoclastic CM or routine medium with recombinant IGF-1 after rapamycin treatment. c Intracardiac injection of 5-8F,5-8F IGF-1R OE or BM3 into mice with/without rapamycin treatment for bone metastasis analysis. Representative BLIs of bone metastases. d BLIs analysis of bone metastases. e Representative micro-CT images of femurs. f Micro-CT analyses for bone destruction of femurs. g Representative H&E images of bone metastases. h H&E analysis of bone metastases. (Tumor tissue is noted in the red line, “ns” indicates P > 0.05, “*” represents P < 0.05, “**” represents P < 0.01,“***” represents P < 0.001 and “****” represents P < 0.0001, Scar bar = 50 μm.)