Fig. 6

CircKEAP1 suppresses OS malignancy through decreasing vimentin levels. (A). OS cells were transfected with circKEAP1 shRNA and control vectors followed by treatment with WFA (5 µM). Control vectors, vimentin or both circKEAP1 and vimentin were transfected into OS cells. Caspase-3 and PARP expression were determined by western blotting. (B). Proliferation of the indicated OS cells was measured by CCK8 assay. (C). Apoptosis of the indicated OS cells was detected by flow cytometry. (D). Migration of the indicated OS cells was quantified by transwell assay. (E). Migration of the indicated OS cells was quantified by wound healing assay. (F). Colony formation ability of OS cells was determined. (G). The indicated cells were injected subcutaneously into nude mice (n = 6 per group) and tumor volume was measured every 7 days. After 28 days, tumors were collected and weighed. Error bars represent three independent experiments. *, **, *** indicates significant differences compared with the control group at a p value < 0.05, < 0.01, < 0.001, respectively