Fig. 3

MUC20 attenuates proteasome inhibitor resistance of MM cells by inducing cuproptosis. A Proliferation rates of control vector and MUC20 OE-transfected PI-resistant KAS-6/1 and U266 cells treated with or without PI (CFZ). B Proliferation rates of PI-sensitive KAS-6/1 and U266 cells treated with or without TTM and PI-resistant KAS-6/1 and U266 cells treated with or without or elesclomol. C Representative images of the flow cytometric analysis for PCD in PI-sensitive KAS-6/1 and U266 cells treated with or without TTM and PI-resistant KAS-6/1 and U266 cells treated with or without or elesclomol. D Proliferation rates of MUC20 OE-transfected PI-resistant KAS-6/1 and U266 cells treated with or without PI (CFZ) and TTM. E Representative images of flow cytometric analysis for PCD in MUC20 OE-transfected PI-resistant KAS-6/1 and U266 cells treated with or without PI (CFZ) and TTM. PS: proteasome inhibitor-sensitive MM cells; PR: proteasome inhibitor-resistant MM cells; OE: overexpression; PI: proteasome inhibitor; TTM: tetrathiomolybdate. *P < 0.05, **P < 0.01, ***P < 0.001