Fig. 4

MUC20 induces cuproptosis through suppressing CDKN2A in proteasome inhibitor-resistant MM cells. A CDKN2A mRNA level in PI-sensitive, PI-resistant, and MUC20 OE-transfected PI-resistant KAS-6/1 and U266 cells. B CDKN2A protein level in PI-sensitive, PI-resistant, and MUC20 OE-transfected PI-resistant KAS-6/1 and U266 cells. C Proliferation rates of MUC20 OE or CDKN2A OE-transfected PI-resistant KAS-6/1 and U266 cells treated with or without PI (CFZ) and elesclomol. D Representative images of flow cytometric analysis for PCD in MUC20 OE or CDKN2A OE-transfected PI-resistant KAS-6/1 and U266 cells treated with or without PI (CFZ) and elesclomol. PS: proteasome inhibitor-sensitive MM cells; PR: proteasome inhibitor-resistant MM cells; OE: overexpression; PI: proteasome inhibitor. *P < 0.05, **P < 0.01