Fig. 8

EccDNA induces proteasome inhibitor resistance by amplifying kinesin family member 3 C to decrease MUC20 expression in MM. A Proliferation rates of control and eccDNA-transfected PI-sensitive KAS-6/1 and U266 cells treated with or without PI (CFZ). B Representative images of flow cytometric analysis for PCD in control and eccDNA-transfected PI-sensitive KAS-6/1 and U266 cells treated with or without PI (CFZ). C Quantification of the volume of xenograft tumors derived from control and eccDNA-transfected PI-sensitive KAS-6/1 and U266 cells in the presence and absence of PI (CFZ). D Images of xenograft tumors formed in nude mice injected with control and eccDNA-transfected PI-sensitive KAS-6/1 and U266 cells in the presence and absence of PI (CFZ). E Quantification of the weights of xenograft tumors formed in nude mice injected with control and eccDNA-transfected PI-sensitive KAS-6/1 and U266 cells in the presence and absence of PI (CFZ). F KIF3C protein level in PI-sensitive and PI-resistant KAS-6/1 and U266 cells. G KIF3C mRNA level in control and eccDNA-transfected PI-sensitive KAS-6/1 and U266 cells. H MUC20 protein level in control and KIF3C-KD-transfected PI-resistant KAS-6/1 and U266 cells. PS: proteasome inhibitor-sensitive MM cells; PR: proteasome inhibitor-resistant MM cells; OE: overexpression; PI: proteasome inhibitor. *P < 0.05, **P < 0.01, ***P < 0.001