Fig. 6

Targeting FAK in combination with PLD induces ICD in vivo and sequentially stimulates antitumor immunity. (A-C) The IHC and IF staining for tumors in the CT26 model generated in BALB/c mice from the efficacy study. Cleaved caspase-3 was stained in the tumors using IHC. Calreticulin and HMGB1 were stained using IF (n = 3 per group). D-F The DCs maturation analysis of the tumors from CT26 models treated for seven days. Flow cytometry was used for the detection (n ≥ 4 per group). G-I The IHC staining of CD3 and CD8 for the tumors from CT26 efficacy study in BALB/c mice. Representative staining images are shown (Scale bar = 20 μm) (G) (n ≥ 3). Arrows indicate the positive staining cells. J-K Serum levels of IFNγ and Granzyme B from the CT26 mice treated with drugs for seven days. The serum was extracted from whole blood for ELISA analysis of different biomarkers (n ≥ 4 per group). In all the experiments above, the positive signal percentages were assessed using ImageJ, and the data were prepared by GraphPad Prism 8.0. Data represent mean ± SEM. Statistical analysis was done using the unpaired student's T-test. *P < 0.05, **P < 0.01, and ***P < 0.001