Fig. 1
From: The synergism of SMC1A cohesin gene silencing and bevacizumab against colorectal cancer
Effects of both SMC1A overexpression and silencing in vitro. A HCT116 transfected with vectors overexpressing or silencing SMC1A. Transfections lead to the overexpression of SMC1A protein or its downregulation when compared to mock cells 24 h after the transfection. Tubulin antibody was used as loading control. B ImageJ software was used to carry out the semiquantitative image analysis of immunoblotting data. C SMC1A silencing affects cell proliferation rate starting from 4th day of in vitro progression. D A normal metaphase (i, mock-treated cell), a tripolar metaphase (ii, SMC1A-Kd cell), a normal anaphase (iii, mock-treated cell), a tripolar anaphase (iv, SMC1A-Kd cell). DNA was stained with DAPI (blue), and mitotic spindle was detected by an anti-α -tubulin antibody (green). E Kinetics of abnormal mitotic figures over 7 days. SMC1A-kd cells show a peak of atypical figures at 4th day of cell culture. *p < 0.05