Fig. 5
From: The synergism of SMC1A cohesin gene silencing and bevacizumab against colorectal cancer
Abnormal mitotic figures and apoptosis in vitro and in vivo. A Tumors induced by shRNA, bevacizumab and combined treatment show high frequency of atypical mitotic figures (mean ± SD). B Morphological features of atypical mitosis. A normal metaphase plate (i), a spontaneous micronucleus (ii, black arrow) and lagging chromosomes (iii, black arrow). These images are representative of combo treatment. C Mitotic abnormal figures are a characteristic of CRC cell lines. Both HT29 and SW620 show high levels of atypical figures. D Apoptotic cells detected by using an anti-Caspase 3 antibody in SMC1A-Ov, shRNA-, bevacizumab- and combo-treated cells. **p < 0.01, *p < 0.05