Fig. 3

Loss of LATS1/2 attenuates the transcriptional responses induced by IFN-γ. (A) Western blotting of indicated proteins in the WCLs from parental and LATS1/2-KO KLE cells treated with TNFα (20 ng/ml) and harvested at different time points. (B) Western blotting of indicated proteins in the WCLs from parental and LATS1/2-KO KLE cells treated with IFN-γ (30 ng/ml) and harvested at different time points. (C) RT-qPCR measurement of the mRNA expression of IFN-γ target genes in parental and LATS1/2-KO KLE cells treated with IFN-γ (30 ng/ml) and harvested at different time points. Data are shown as means ± SD (n = 3). (D, E) ChIP-qPCR measurement of the enrichment of STAT1 in NLRC5 (D) and IRF1 (E) promoter regions treated with IFN-γ (30 ng/ml) for 6 h. P values are calculated using the Multiple t-tests in (C) and One-way ANOVA test in (D, E). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001