Fig. 5
Increased resistance to 5-FU after prior exposure to chemotherapy. The association between prior chemotherapy exposure to patients before the biopsy for PDOs was established and organoid response (GRAUC) is shown for 5-FU, oxaliplatin and SN-38. A, C and D. The DRC of organoid sensitivity with red curves indicating 5-FU or capecitabine containing chemotherapy exposure. B, D and F Boxplots of normalized GRAUC for patients that were exposed to chemotherapy versus chemotherapy-naïve patients. Boxplots show the minimum, median, maximum, upper and lower quartiles and individual data points. Abbreviations: 5-FU (5-flouruoracil), GRAUC (area under the growth rate inhibition curve), SN-38 (active metabolite of irinotecan)