Table 2 Comparison of organoid drug screening conditions

Screening medium 5-FU & oxaliplatin

With NAC (CQ ratio, GR)

r = 0.32 [− 0.78, 0.94]

Without NAC (CQ ratio, GR)

r = 0.77 [− 0.35, 0.98]

Readout 5-FU & oxaliplatin

CQ (ratio without NAC, GR)

r = 0.77 [− 0.35, 0.98]

CTG (ratio without NAC, GR)

r = 0.81 [− 0.24, 0.99]

Drug response curve metrics: Viability vs GR 5-FU & oxaliplatin

Viability (ratio without NAC)

r = 0.46 [− 0.19, 0.83]

GR (ratio without NAC)

r = 0.6 [− 0.01, 0.88]

Drug response curve metrics: Viability vs GR 5-FU & SN-38

Viability (CTG fixed)

r = 0.26 [− 0.54, 0.82]

GR (CTG fixed)

r = 0.66 [− 0.08, 0.93]

Drug response curve metrics: Viability vs GR 5-FU

Viability (CTG)

r = 0.3 [− 0.68, 0.89]

GR (CTG)

r = 0.58 [− 0.44, 0.95]

Combination treatment set-up 5-FU & oxaliplatin

Fixed (CTG with NAC, GR)

r = − 0.25 [− 0.76, 0.45]

Ratio (CQ without NAC, GR)

r = 0.6 [− 0.01, 0.88]

Combination treatment set-up 5-FU & SN-38

Fixed (CTG, GR)

r = 0.66 [− 0.08, 0.93]

Ratio (CTG, GR)

r = 0.3 [− 0.79, 0.94]