Fig. 2

Antiproliferative effects of RC48 and CM alone on OC cells. A, B A2780, OVCAR-3, SK-OV-3, HO-8910PM and UM-UC-3 cells were treated with RC48, T-DM1, T-DXd and trastuzumab for 72 h, respectively. Cell viability was detected using Cell Titer-Glo cytotoxicity assays. C The proliferation of OC cells treated with RC48 for 60 h was observed using the Incucyte real-time cell analysis system. D-G The anti-tumor activity and survival benefit of 5 and 10 mg/kg RC48 were evaluated in the OC-52 (D, E) and OC-94 (F, G) PDX models. H The toxic effects of VEGFR inhibitors on A2780 cells were evaluated, and a scatter map of MIR-IC50 was generated. I A2780, OVCAR-3, SKOV-3 and HO-8910PM cells were treated with CM for 72 h, and cell viability was detected using Cell Titer-Glo cytotoxicity assays. J The proliferation of OC cells treated with CM for 60 h was observed using the Incucyte real-time cell analysis system. Data represent the mean ± standard error of the mean (SEM) of at least three independent experiments, and statistical significance was assessed by unpaired T-test (***p < 0.001)