Fig. 7

Synergistic inhibition of tumor growth in OC CDX models by RC48 and CM. In both CDX models, mice were intravenously administered with VEH, 2.0 mg/kg (MPK) RC48 (once a week for 3 times), 0.5 MPK CM (once every day), or COM. A, B Tumor growth curve and transplanted tumor weight were evaluated in the A2780 (A) and OVCAR-3 (B) CDX models. C, D Representative IHC image of Ki‐67 staining (a proliferation index) in A2780 (upper panel) and OVCAR-3 (lower panel) xenograft tumors. Images were captured using TissueFAXS Plus at 400 × magnification. Scale bars = 20 µm. E, F The quantification of apoptotic cells in A2780 (upper panel) and OVCAR-3 (lower panel) xenograft tumors was determined using TUNEL assay. Apoptotic cells are represented by green fluorescent-stained cell nuclei. Images were captured using LSCM at 400 × magnification. Scale bars = 20 µm. The data shown represent the mean ± SEM from three independent experiments performed in triplicate. All treated groups compared to VEH group or COM group compared to single agent group. Statistically significant values are denoted as *p < 0.05, **p < 0.01, and ***p < 0.001