Fig. 2

Hypoxia and PLD2 modify the chromatin landscape of OC cells. (A) Differential analyses of accessibility between OC cells carrying Ev under normoxic or hypoxic conditions from ATAC-seq data (n = 2 biological replicates per condition). The log2 normalized read counts of peaks versus the log2-fold-change of accessibility are plotted. Peaks showing a statistically significant change (P value < 0.05) are highlighted in blue (hypoxia increased peaks) or red (normoxia increased peaks). (B) Heatmaps plotting normalized ATAC-seq signals at differentially accessible regions (DARs) from (f) in OC cells carrying Ev or expressing PLD2 under normoxia and carrying Ev or expressing shPLD2 under hypoxia. (C) Differential transcription factor (TF) binding analysis in OC cells carrying Ev under normoxia vs. hypoxia conditions using TOBIAS. Volcano plot showing the differential binding score versus the -log₁₀ p value. TF motifs with increased (blue) or decreased (red) binding in hypoxia are highlighted. (D) Clustering of TFs with increased (top) or decreased (bottom) binding in hypoxia. (E) Tracks with ATAC-seq signals in OC cells carrying Ev in hypoxia or normoxia, expressing PLD2 in normoxia or expressing shPLD2 in hypoxia at the SOX9, PROM1, and WNT7A loci