Fig. 1

Annotation and function of myeloid cells and macrophages by scRNA-seq in tumor and normal tissue. a UMAP plot colored by different clusters. b Dot plot of mean expression of myeloid cells and macrophages’ characteristic genes in different clusters. c UMAP plot colored by different cell types. d Complex heatmap of selected marker genes in each cell cluster. Up: Tissue preference of each cluster. Down: Relative expression of marker genes associated with each cell subset. e Relative contribution of each cell type in normal vs. tumor tissue and early vs. advanced LUAD. f Kaplan–Meier curves of survival analysis in TCGA LUAD patients based on the TAMs infiltration. g Left: Difference genes between normal tissue, early LUAD, and advanced LUAD, with a threshold of |log2FC| > 0.5 & p_val_adj < 0.05, and take the intersection of the differential genes of the three groups. Right: Heatmap of 26 genes that differ among the three groups. h Immunofluorescence shows the expression of TAMs marker and IL-6 (Red CD68, Pink CD163, Green IL-6, Blue Dapi), scale bar represents 50 μm