Fig. 2

TAMs promote LUAD cell proliferation, migration and invasion by secreting IL-6. a Cell migration and invasion ability of LUAD cells (A549 and H358) alone, stimulated with IL-6 or stimulated with IL-6 followed by addition of IL-6 neutralizing antibody was determined by the transwell assay. b Cell proliferation ability of LUAD cells (A549 and H358) alone, stimulated with IL-6 or stimulated with IL-6 followed by addition of IL-6 neutralizing antibody was determined by the CCK8 assay. c The tumor size and tumor weight in the A549 alone, A549 + IL-6 stimulation and A549 + IL-6 stimulation + IL-6-NA groups. d RT-qPCR analyzed the expression of the most often released cytokines by macrophages after IL-6 stimulation. e Elisa analysis of IL-6 showed changes in macrophage IL-6 expression over time after IL-6 stimulation. f & g Cell migration, invasion and proliferation ability of LUAD cells (A549 and H358) alone, co-culture with M2-like macrophages or co-culture with M2-like macrophages and stimulated with IL-6 was determined by the transwell assay and CCK8 assay