Fig. 3

IL-6 promotes LUAD progression by activating the JAK2/STAT3 pathway in M2-like macrophages. a GSVA analysis of differential pathways between AMs, TAMs in early and advanced LUAd. b Effects of IL-6 stimulation on common pathway expression in TAMs detected by western blot analysis. c Expression of the JAK2/STAT3 pathway with IL-6-stimulated, IL-6-stimulated with the addition of IL-6-NA in M2-like macrophages were analyzed by western blot. d Effect of AZD1480 and WP1066 on JAK2/STAT3 pathway expression in THP1 derived M2-like macrophages after IL-6 stimulation were analyzed by western blot. e Cell migration and invasion ability of LUAD cells alone, co-culture with M2-like macrophages or co-culture with M2-like macrophages followed by addition of AZD1480 and WP1066 was determined by the transwell assay. f RT-qPCR and Elisa analysis showed IL-6 expression in M2-like macrophages after IL-6 stimulation or IL-6 stimulation with the addition of AZD1480 and WP1066 (Left: Elisa results. Right: RT-qPCR results). g IL-6 expression after STAT3 overexpression or knockdown was analyzed by western blot. h RT-qPCR and Elisa analysis showed IL-6 expression in M2-like macrophages after STAT3 overexpression or knockdown