Fig. 4
pSTAT3 promotes tumor progression by regulating C/EBPβ expression in TAMs. a Cell migration and invasion ability of LUAD cells, C/EBPβ overexpression or C/EBPβ knockdown was determined by the transwell assay. b Effects of IL-6 stimulation on C/EBPβ expression in TAMs. c Expression of C/EBPβ after STAT3 overexpression or knockdown. d RT-qPCR and Elisa analysis showed IL-6 expression in M2-like macrophages after C/EBPβ overexpression or knockdown. e Expression of pSTAT3 and C/EBPβ in the nucleus after IL-6 stimulation. f The localization of pSTAT3 and C/EBPβ were analyzed by immunofluorescence staining. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue). The line charts represent fluorescence intensity (MFI), presenting the distance from α to β, scale bar represents 100 μm