Fig. 6

Mechanisms of STAT3 regulation of C/EBPβ and C/EBPβ regulation of IL-6. a ChIP assay demonstrated the binding of STAT3 to the C/EBPβ promoter in THP1-derived M2-like macrophages. b RT-qPCR of the ChIP products confirmed the binding capacity of STAT3 to the C/EBPβ promoter. c Selective mutation analyses identified STAT3-responsive regions in the C/EBPβ promoter. d Luciferase reporter plasmids with wild and mutated C/EBPβ promoters were transfected into 293 T cells. Relative luciferase activity was determined using IL-6 stimulation or STAT3 overexpression. e ChIP assay demonstrated the binding of C/EBPβ to the IL-6 promoter in. f RT-qPCR of the ChIP products confirmed the binding capacity of C/EBPβ to the IL-6 promoter. g Selective mutation analyses identified C/EBPβ-responsive regions in the IL-6 promoter. h Luciferase reporter plasmids with wild and mutated IL-6 promoters were transfected into 293 T cells. Relative luciferase activity was determined using IL-6 stimulation, C/EBPβ or STAT3 overexpression