Fig. 7
IL-6 promotes LUAD migration and invasion through activation of EMT pathway in vivo and in vitro. a Expression of EMT-related genes after co-culture with M2-like macrophages. b Expression of EMT-related genes using IL-6 stimulation or IL-6-neutralising antibodies. c IHC analyzed the expression of CD206, IL-6, E-cadherin, N-cadherin, Vimentin and Snail, and their relationships of tumors from LUAD patients. d The tumor size and tumor weight in the A549 alone, A549 + control TAMs, A549 + IL-6 overexpression TAMs, A549 + IL-6 knockout TAMs, and A549 + TAMs + IL-6 neutralizing antibodies groups. e IHC analyzed the expression of E-cadherin, N-cadherin, Snail and Vimentin protein of tumors from the subgroups above. Scale bar represents 10 μm