Fig. 5From: Tumor-derived exosomal ADAM17 promotes pre-metastatic niche formation by enhancing vascular permeability in colorectal cancerKnocking down exosomal ADAM17 effectively reduces colorectal cancer (CRC) metastasis in vivo. (a) Schematic showing the tumor metastasis experiments used to assess EMT-HCT116-Exo-treated mice (Si-NC and Si-ADAM17 transfected). (b) Representative immunohistochemistry staining for VE-cadherin and immunofluorescence staining of CD34/VE-cadherin at the invasive front in mice with CRC (black scale bar = 50 μm, white scale bar = 25 μm). (c) Relative VE-cadherin level at the invasive front (n = 10) and CTC counts (n = 20) in EMT-HCT116-Exo-treated mice (Si-NC and Si-ADAM17 transfected). (d) Representative H&E staining results of lung sections from EMT-HCT116-Exo-treated mice (Si-NC and Si-ADAM17 transfected). Arrows indicate tumor nodules. Scale bar = 250 μm. (e) Evans blue accumulation in the lung parenchyma (n = 8). (f) Percentage of mice with lung metastasis, alongside the number and maximal diameter of metastatic nodules in the lungs of EMT-HCT116-Exo-treated mice (Si-NC and Si-ADAM17 transfected). (g) Representative H&E staining results of liver sections from EMT-HCT116-Exo-treated mice (Si-NC and Si-ADAM17 transfected). Arrows indicate tumor nodules. Scale bar = 250 μm. (h) Evans blue accumulation in the liver parenchyma (n = 8). (i) Percentage of mice with liver metastasis, alongside the number and maximal diameter of metastatic nodules in the liver of EMT-HCT116-Exo-treated mice (Si-NC and Si-ADAM17 transfected). Data are expressed as mean ± standard deviation. *P < 0.05, **P < 0.01, compared with the control group; #P < 0.05, ##P < 0.01, compared with the Si-NC groupBack to article page