Fig. 1

Screening of hub SE-associated gene IGF2BP2 in HNSCC. A The ROSE algorithm was utilized to plot the enhancer regions of normal oropharyngeal mucosa and HNSCC cell lines CAL27, Detroit562, HN12, and SCC25 in increasing order based on the H3K27Ac ChIP-seq signal, and enhancers above the inflection point of curve are defined as SEs. B In a comprehensive comparison of these HNSCC cell lines, 771 specific SE-associated genes and 442 specific SE-associated protein-encoding genes (mRNA) were identified, compared to SE-associated genes in normal oropharyngeal mucosa. C Disease-gene network enrichment analysis revealed that HNSCC cell-specific SE-associated genes were most enriched in carcinoma of the larynx. D-F WGCNA analysis identified the most relevant modules in the TCGA-HNSCC (D), GSE30784 (E), and GSE42743 (F) datasets for HNSCC, which were represented by the green (r = 0.47), turquoise (r = 0.74), and black (r = 0.71) modules, respectively. G Wayne diagram depicted the intersection of HNSCC cell-specific SE-associated protein-encoding genes with HNSCC-associated genes obtained from TCGA-HNSCC, GSE30784, and GSE42743 in WGCNA