Fig. 4

IGF2BP2 is a SE-driven gene. A Visualization of H3K27Ac level of IGF2BP2 with IGV Genome Browser. B and C Western blot (B) and qRT-PCR (C) were used to detect the expression of IGF2BP2 after the blockade of the IGF2BP2-SE region by CRISPR/Cas9 gene editing. D and E Western blot was used to detect the expression of IGF2BP2 after silencing BRD4 (D) or MED1 (E). F–H QRT-PCR was performed to assess the relative IGF2BP2 expression (H) after silencing BRD4 (F) or MED1 (G). I Silencing BRD4 reduced the BRD4 enrichment in the IGF2BP2-SE region. J Silencing MED1 reduced the MED1 enrichment in the IGF2BP2-SE region. K and L Detection of IGF2BP2 protein level and mRNA expression by Western blot (K) and qRT-PCR (L) after THZ1, JQ1, OTX-015, and CPI-637 treatment. M ChIP-qPCR showed that the enrichment of BRD4 in IGF2BP2-SE was reduced by treating with JQ1. N ChIP-qPCR showed that the enrichment of H3K27Ac in IGF2BP2-SE was reduced by treatment with CPI-637. *P < 0.05, **P < 0.01, ***P < 0.001. All the data are presented as mean ± SD from three independently performed experiments