Fig. 5

BRD4 inhibition with JQ1 impairs HNSCC progression in vitro and in vivo. A CCK-8 assay was used to detect the proliferation of SCC25 and CAL27 cells after treatment with JQ1. B Clone formation assay detected the clonal proliferation of SCC25 and CAL27 cells after treatment with JQ1. C The migration and invasion of SCC25 and CAL27 cells after treatment with JQ1 were photographed and measured. Scale bar, 100 μm. D Images of the tongue of BALB/c-Nude mice after injection of CAL27 cells and treatment with control vehicle or JQ1. E Representative images of tumor tissues after H&E staining. Scale bar, 1 mm. F Comparison of tumor volumes treated with control vehicle or JQ1. G Fisher's exact test analysis of the percentage of CLN metastasis. H Representative immunohistochemical staining images for Pan-Keratin in the CLN. Scale bar of the upper panel, 500 μm. Scale bar of the lower panel, 200 μm. I Representative images of H&E staining, BRD4 IHC staining, and IGF2BP2 IHC staining in HNSCC orthotopic xenografts treated with control vehicle or JQ1. Scale bar of the left panel, 300 μm. Scale bar of the right panel, 200 μm. *P < 0.05, **P < 0.01, ***P < 0.001. All the data are presented as mean ± SD from three independently performed experiments