Fig. 6

SE-associated IGF2BP2 is transcriptionally activated by KLF7. A Wayne diagram depicted the intersection of transcription factors bound to IGF2BP2-SE and promoter. B-E Correlation of IGF2BP2 expression with KLF7 in four HNSCC datasets, TCGA-HNSCC (B), GSE30784 (C), GSE42743 (D), and GSE41613 (E). F–H TCGA-HNSCC (F), GSE30784 (G), and GSE42743 (H) datasets revealed that KLF7 expression was upregulated in HNSCC. I and J Poor overall survival in patients with high KLF7 expression in the TCGA-HSCCC (I) and GSE42743 (J) datasets. K Western blot was used to assess the protein level of IGF2BP2 after silencing KLF7. L and M QRT-PCR detected the relative IGF2BP2 expression (M) after silencing KLF7 (L). N Western blot was performed to detect the protein level of IGF2BP2 after stable overexpression of KLF7. O and P QRT-PCR were used to assess the relative IGF2BP2 expression (P) after stable overexpression of KLF7 (O). Q Prediction of KLF7 binding sites and binding motifs in IGF2BP2-SE and promoter regions using the JASPAR website. R Binding sites and binding motifs of KLF7 in the SE region of the IGF2BP2 coding chain. S Schematic representation of wild-type and mutant KLF7 binding motifs in IGF2BP2-SE region. T Reduced luciferase activity after mutating the binding site of KLF7 to IGF2BP2-SE region. U The bindings of KLF7 to the IGF2BP2-SE and promoter regions were examined by ChIP-qPCR. *P < 0.05, **P < 0.01, ***P < 0.001. All the data are presented as mean ± SD from three independently performed experiments