Fig. 1

Inflammatory profiles of AT-3 mammary tumors and B16F1 melanoma. AT-3 and B16F1 tumors were grown in C57BL/6 mice for 21 days and 14 days, respectively. (A) Comparative heatmap showing cytokines and chemokines expressed in AT-3 and B16F1 tumors using Proteome Profiler array (n = 4 tumors, p < 0.05). (B) Representative images and quantification of CD45 staining in AT-3 and B16F1 tumors. n = 8 AT-3 and 6 B16F1 tumors, each dot represents the mean value calculated from 5 random images taken with a 10x objective per tumor. (C) Comparison of granzyme B levels in AT-3 and B16F1 tumor extracts. (n = 32 AT-3 and 25 B16F1 tumors). (D) Scatter plot of Spearman’s rank correlation between tumor weight and granzyme B in AT-3 (n = 32) and B16F1 tumors (n = 25). (E) Comparison of PD-L1 levels in AT-3 (n = 19) and B16F1 tumors (n = 14)