Fig. 2

Angiogenic profiles of AT-3 mammary tumors and B16F1 melanoma. (A) Comparative heatmap of angiogenic factors expressed in AT-3 and B16F1 tumor extracts using Proteome Profiler array (n = 4 tumors, p < 0.05). (B) Representative images of collagen IV staining in AT-3 and B16F1 tumors. (C) Quantification of blood vessels identified by collagen IV staining in AT-3 and B16F1 tumors. n = 8 AT-3 and 6 B16F1 tumors, each dot represents the mean value calculated from 5 random images taken with a 10x objective per tumor. (D) Quantification of mean vessel perimeter in AT-3 and B16F1 tumors. n = 8 AT-3 and 6 B16F1 tumors; each dot represents the mean value calculated from 10 random images taken with a 10x objective per tumor. (E) Tumor vascular perfusion as assessed by measurement of intratumor FITC-dextran content of B16F1 and AT-3 tumors explanted 5 min after intravenous injection of FITC-dextran, 150 kDa. (n = 27 AT-3 and 20 B16F1 tumors). (F) H&E staining of both tumor types showing tumor bleeding only in B16F1 tumors. Arrows indicate intratumor hemorrhage. Comparison of intratumor hemoglobin content between AT-3 (n = 32) and B16F1 tumors (n = 25). ns, non-significant