Fig. 6

Extravascular platelet clusters in AT-3 tumors from chronic and severe thrombocytopenic mice. (A) Representative image of immunostaining for platelet factor 4 (PF4) in mammary AT-3 tumors from mice with chronic severe thrombocytopenia (c-mpl−/− mice treated with R300 antibody). Note the abundance of platelet clusters at the tumor periphery. (B) Immunostaining for platelets (PF4, VWF, GPIX) showing the presence of extravascular platelet clusters in AT-3 tumors from mice with chronic severe thrombocytopenia. White boxes show higher magnification views of the squared areas