Fig. 8

Impact of thrombocytopenia on vascular permeability and endothelial integrity in B16F1 and AT-3 tumors. A. Representative images of the macroscopic aspects of B16F1 and AT-3 tumors from control mice and mice with chronic severe thrombocytopenia, with or without intravenous injection of FITC-dextran, 2000 kDa prior to sacrifice. AT-3 tumors in control mice are highlighted by white arrows. B-C. Representative images and corresponding endothelial integrity scores of PECAM-1 (B) and VE-cadherin (C) staining for evaluation of endothelial integrity in AT-3 and B16F1 tumors from control mice and mice with chronic severe thrombocytopenia. Maximal intensity projections of optical sections are shown. D. Comparison of soluble PECAM-1 levels in plasma from control mice and mice with chronic severe thrombocytopenia with AT-3 (platelet 100%, n = 8; <10%, n = 5) or B16F1 (platelet 100%, n = 21; <10%, n = 12) tumors. ns: non-significant