Fig. 1
The CM of Gli1-overexpressing NSCLC cells promoted angiogenesis in vitro and ex vivo. A Flowchart of evaluation the effect of Gli1-overexpressing A549 and NCI-H460 cells on endothelial cell and aorta rings. B-E The effect of CM from A549Gli1 vector and NCI-H460Gli1 vector cells on the migration, invasion and tube formation abilities of HUVECs and HMEC-1 cells. HUVECs and HMEC-1 cells were treated with CM for 48 h and subjected for wound healing (B), Transwell migration (C) and Tiranswell invasion (D), and tube formation assays (E). F Gli1-overexpressing NSCLC increased blood sprouting in aortic ring assay. G The CM of Gli1-overexpressing cells promoted the HBVP adhesion ability. H Schematic strategy of coculture tubule-like structure of endothelial cells and pericytes. I CM from Gli1-overexpressing cells enhanced the recruitment of HBVP to tubes formed by endothelial cell. All the data were showed as mean ± SD, n = 3. **p < 0.01; ***p < 0.001 vs A549NC vector or NCI-H460NC vector cells