Fig. 3

The CM from Gli1-knockdown NSCLC cells suppressed angiogenesis in vitro and ex vivo. A Schematic strategy of endothelial cells and aorta rings treated with CM from Gli1-knockdown NSCLC cells. B-E The CM from NCI-H1299^shGli1 cells or NCI-H1703^shGli1 cells suppressed the migration (B and C), invasion (D) and tube formation (E) abilities of HUVECs and HMEC-1 cell. F The CM from NCI-H1299^shGli1 cells or NCI-H1703^shGli1 cells reduced vessel sprouting in the rat aorta rings. G-I The CM from NCI-H1299^shGli1 cells and NCI-H1703^shGli1 cells inhibited HBVP adhesion (G) and recruitment to newly blood vessel (H and I). All the data were showed as mean ± SD, n = 3. ^**p < 0.01; ^***p < 0.001 vs NCI-H1299^{sh NC} or NCI-H1703^{sh NC} cells