Fig. 4

Genetic inhibition of Gli1 attenuated tumor growth and tumor angiogenesis. A Flowchart of the protocol to evaluate whether Gli1-knockdown NCI-H1299 or NCI-H1703 cells affect tumor angiogenesis and pericyte coverage. B, C Gli1-knockdown NCI-H1299 cells attenuated tumor growth. The tumor volume (B) and tumor weight statistics (C) were showed. D, E Gli1 silencing in NCI-H1299 cells induced large necrotic area, less Ki67 positive cells and reduced microvessel density in xenograft. The representative images of IHC and quantitative data were showed in (D) and (E). F, G Genetic inhibition of Gli1 in NCI-H1299 cells reduced pericyte coverage and dextran leakage in xenograft tissues, n = 7. H-N Gli1 knockdown in NCI-H1703 cells suppressed tumor growth (H-J), decreased microvessel density (K and L) and reduced pericyte coverage of blood vessel (M), and dextran leakage (N). All the data were showed as mean ± SD, n = 6. ^**p < 0.01; ^***p < 0.001 vs NCI-H1299^{sh NC} or NCI-H1703^{sh NC} cells