Fig. 5
bFGF was involved in Gli1-mediated enhancement effect on tumor angiogenesis. A Human angiogenesis array analysis of CM from A549NC vector or A549Gli1 vector cells. And the red box indicates the 8 significant up-regulated protein in CM of A549Gli1 vector cells when compared with that of A549NC vector cells. B Summary of Human angiogenesis array analysis of relative signal intensity of upregulated cytokines. C The bFGF protein level in the CM of four paired Gli1-overexpressing or Gli1-knockdown cells were detected by ELISA assay, n = 3. D Western blotting detected the bFGF expression in 4-paired Gli1-overexpressing or Gli1-knockdown NSCLC cells, n = 3. E–F The bFGF expression in 80-paired NSCLC tumor tissues and their corresponding noncancerous lung tissues were detected by IHC. The representative images were showed in (E) and the statistical data were displayed in (F). ***p < 0.001 vs adjacent tissues. G RT‒qPCR assay determined the bFGF mRNA in 11-paired NSCLC tumor tissues and their corresponding adjacent tissues. ***p < 0.001 vs adjacent tissues. H Kaplan–Meier analysis of NSCLC patients with a high bFGF expression and a low bFGF expression. I-J bFGF expression in xenograft tumors of 2-paired Gli1-overexpressing NSCLC cell lines and 2-paired Gli1-knockdown NSCLC cells detected by IH. All the data were showed as mean ± SD. ***p < 0.001 vs NC vector group or shNC group