Fig. 7

Gli1 regulated bFGF expression by enhancing bFGF transcriptional activity and reducing bFGF protein degradation. A Gli1 positively regulated bFGF mRNA level. ^***p < 0.001 vs NC vector group or sh NC group. B Gli1 enhanced the luciferase of bFGF promoter. ^***p < 0.001 vs NC vector group. C Dual-luciferase assay showed that Gli1 regulated bFGF expression by binding to its promoter. ^**p < 0.01, ^***p < 0.001vs P0WT group. D The result of ChIP-PCR assay. ^**p < 0.01, ^***p < 0.001vs IgG group. E The effect of Gli1 on bFGF protein stability. Cells were treated with CHX (2 μM) for 0–6 h, and the cells were collected and subjected for Western blotting. ^***p < 0.001vs NC vector or shNC group. F The effect of Gli1 on the degradation of bFGF. G The effect of Gli1 on the ubiquitinations of bFGF. All the data were showed as mean ± SD, n = 3