Fig. 8
GANT-61 suppressed tumor angiogenesis. A-C The effect of GANT-61 on the migration (A), invasion (B) and tube formation abilities (C) of endothelial cells. D The effect of GANT-61 on the vessel formation measured by aortic ring assay. n = 3. E–H The effect of GANT-61 on the tumor growth and angiogenesis. When the tumor volume reached approximately 50 mm3, the tumor bearing mice were randomly intraperitoneally injected with vehicle or GANT-61 (25 mg/kg) every two day for a total of 24 days. At the end of the experiment, the mice were sacrificed and the tumors were removed, weighted and photographed. The tumor volume curve and the images of tumors were showed in (E), the tumor weight statistics was presented in (F), the representative images of IHC assay and the quantitative data were displayed in (G) and (H). I, J The effect of GANT-61 on the pericyte coverage of blood vessel in NCI-H1299 xenografts. K, L The effect of GANT-61 on the dextran leakage of tumor vascular. All the data were showed as mean ± SD, n = 6. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the vehicle group