Fig. 1

CircSLCO1B3 is highly expressed and correlated with poor prognosis in ICC patients. a Cluster heat map of the representative results of circRNA-seq. b The expression level of circRNAs with significantly high expression in CCA tissues were detected by qPCR in 13 CCA tissues and matched normal tissues. c FISH scores statistical analysis of circSLCO1B3 in ICC tissues and normal tissues (n=93). d, e Kaplan-Meier analyses of the correlation between circSLCO1B3 expression and overall survival or recurrence. f, g, h, i FISH images of circSLCO1B3 in ICC tissues and normal tissues (circSLCO1B3-FISH: green spots, DAPI: blue spots) (magnification, × 200, scale bar, 50 μm). j Schematic illustration of the genomic location and back splicing of circSLCO1B3, with the splicing site validated by sanger sequencing. k Convergent and divergent primers were employed to confirm the closed loop topology of circSLCO1B3. l qPCR analysis of circSLCO1B3 and linear SLCO1B3 in ICC cells treated with Actinomycin D at the indicated time point. m, n The subcellular location of circSLCO1B3 in RBE cells by FISH (DAPI: blue spots) (magnification, × 400, scale bar, 20 μm and magnification, × 1000, scale bar, 10 μm). (Values are expressed as the means ± SDs; *P < 0.05, **P < 0.01 and ***P < 0.001)