Fig. 2

CircSLCO1B3 promoted proliferation, migration and invasion of ICC cells in vitro. a, b, c, d qPCR analysis of circSLCO1B3 and linear SLCO1B3 expression in ICC cells transfected with circSLCO1B3 siRNAs or plasmids. e, f The growth curves of ICC cells were evaluated by CCK-8 assays. g The colony formation assays showed the cell colony number after knocking down and overexpressing circSLCO1B3 in CCA cells. h, i EdU assays of CCA cells were performed to evaluate cell proliferation (magnification, × 200, scale bar, 100μm). j, k, l, m, n, o, p, q Trans-well assays and wound healing assays were conducted to determined cell migration and invasion (magnification, × 200, scale bar, 100 μm). r, s qPCR and western blotting assays to examine the effect of circSLCO1B3 on epithelial and mesenchymal marker expression. Data were showed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001