Fig. 3

CircSLCO1B3 promoted ICC progression via inhibiting expression of miR-502-5p. a, b, c, d RIP assays using an anti-Ago2 antibody and IgG were performed in RBE and HuCCT1 cells. e Schematic illustration exhibiting overlapping of the target miRNAs of circSLCO1B3 predicted by miRDB database, targetscan database and microRNA-seq results. f, g RNA pull-down was executed in RBE and HuCCT1 cells to determine the relative expression levels of 12 potential target miRNAs by the circSLCO1B3 probe. h, i qPCR evaluated the expression of miR-502-5p after orchestrating circSLCO1B3 in ICC cells. j, k, l qPCR determined the expression of miR-502-5p in ICC tissues and cells. m The cellular locations of circSLCO1B3 (green) and miR-502-5p (red) in RBE cells were examined using FISH assays (magnification, × 400, scale bar, 20 μm and magnification, × 1000, scale bar, 10 μm). n Schematic illustrations and the relative luciferase activities of circSLCO1B3-WT and circSLCO1B3-MUT luciferase reporter vectors. o, p Pull-down was executed in ICC cells to detect the enrichment of circSLCO1B3 by the miR-502-5p probe. q, r, s, t, u, v Wound healing assays and colony formation assays were conducted to determine the migratory and proliferative capabilities in ICC cells transfected with miR-502-5p mimics and inhibitors. Data were showed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001